BrdU antibody
| Antigen |
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| Clonality |
Monoclonal (BrdU 206) |
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Host
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Reactivity
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Compound (28), Human (13), Mouse (Murine) (12), Rat (Rattus) (12), Dog (Canine) (5), Rabbit (4), Sheep (Ovine) (4), Various Species (4), Chicken (3), Cow (Bovine) (3), Pig (Porcine) (3), Horse (Equine) (1)
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Application
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 Alternatives Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (51), Immunofluorescence (IF) (41), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), ELISA (6), Immunohistochemistry (IHC) (6), Western Blotting (WB) (6), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Immunocytochemistry (ICC) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN336860 |
| Quantity |
7 ml (Variants) |
| Price |
100.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Application Notes
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IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. (For staining of FFPE tissue sections, incubate with 4 N HCl for 20 minutes at RT, followed by treatment with proteolytic digestion for 10 minutes at RT. (Please refer to our Trypsin (AR-6541), Pronase (AR-6542) or pepsin (AR-6543) data sheets. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Liver of experimental animal injected with BrdU or cell line grown in presence of BrdU. Cellular Localization: Nuclear.
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Buffer
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The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a syn
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Storage
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2-8°C
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Restrictions
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For Research Use only
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Alternatives for antigen "BrdU", type "Antibodies"
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Hosts
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Rabbit (41), Mouse (13), Rat (6), Sheep (2)
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Reactivities
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Compound (28), Human (13), Mouse (Murine) (12), Rat (Rattus) (12), Dog (Canine) (5), Rabbit (4), Sheep (Ovine) (4), Various Species (4), Chicken (3), Cow (Bovine) (3), Pig (Porcine) (3), Horse (Equine) (1)
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Applications
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Flow Cytometry (FACS) (51), Immunofluorescence (IF) (41), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), ELISA (6), Immunohistochemistry (IHC) (6), Western Blotting (WB) (6), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Immunocytochemistry (ICC) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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FITC (7), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Alexa Flour 488 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
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