S-100 antibody
| Antigen |
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| Clonality |
Monoclonal (S100-217) |
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Host
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Reactivity
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Alternatives Human, Monkey, Mouse (Murine), Rat (Rattus), Cow (Bovine)
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Application
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Alternatives Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN336901 |
| Quantity |
7 ml (Variants) |
| Price |
100.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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S100
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Immunogen
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Purified bovine S100 protein
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Format
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Liquid (prediluted)
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Isotype
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IgG1, kappa chain (Matching secondary antibodies)
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Clone
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S100-217
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Specificity
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Molecular Weight: ~22 kDa.
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Application Notes
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IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Melanoma or schwannoma. Cellular Localization: Cytoplasmic.
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Buffer
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The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description S100 protein is a type of low molecular weight protein fo
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Research Area
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Cell Cycle
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Restrictions
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For Research Use only
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Alternatives for antigen "S-100", type "Antibodies"
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Hosts
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Rabbit (24), Mouse (15), Goat (1)
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Reactivities
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Human (29), Cow (Bovine) (23), Mouse (Murine) (18), Rat (Rattus) (18), Monkey (10), Chicken (1), Kangaroo (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Immunohistochemistry (IHC) (12), Western Blotting (WB) (7), Flow Cytometry (FACS) (6), Immunofluorescence (IF) (6), ELISA (3), To be determined by user (TBD) (2)
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Conjugates
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FITC (4)
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