AGAP3 antibody (AA 899-911)

Catalog No. ABIN337013

The Goat Polyclonal anti-AGAP3 antibody is suitable to detect AGAP3 in samples from Human, Hamster, Monkey and Rabbit. It has been validated for ELISA, IHC and IHC (p).

Quick Overview for AGAP3 antibody (AA 899-911) (ABIN337013)

Target: AGAP3 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3))

Reactivity: Human, Hamster, Monkey, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This AGAP3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AGAP3 Antibody

Binding Specificity: AA 899-911

Specificity: Human AGAP3 / CENTG3.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Rabbit (100%) Mouse, Rat, Bat, Bovine, Elephant, Panda, Horse (92%) Dog, Opossum (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NPSAELHRSPSLL from the C-terminus of human AGAP3 / CENTG3 (NP_114152.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Rabbit (100%), Mouse, Rat, Bat, Bovine, Elephant, Panda, Horse (92%), Dog, Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AGAP3

Target: AGAP3 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3))

Alternative Name: AGAP3 / CENTG3 / MRIP-1

Background: Name/Gene ID: AGAP3

Synonyms: AGAP3, AGAP-3, Centaurin, gamma 3, Centaurin-gamma-3, CRAG, CRAM-associated GTPase, MRIP-1, CENTG3, Cnt-g3, MR1-interacting protein

Gene ID: 116988

NCBI Accession: NP_114152

UniProt: Q96P47

Pathways: Protein targeting to Nucleus