MPP5 antibody (AA 174-187)

Catalog No. ABIN337015

This Goat Polyclonal antibody specifically detects MPP5 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Dog, Cow, Horse, Rabbit, Pig and Monkey.

Quick Overview for MPP5 antibody (AA 174-187) (ABIN337015)

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Reactivity: Human, Dog, Cow, Horse, Rabbit, Pig, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This MPP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MPP5 Antibody

Binding Specificity: AA 174-187

Specificity: Human MPP5.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Bovine, Dog, Elephant, Panda, Horse, Rabbit, Pig (100%) Bat, Hamster, Turkey, Chicken (93%) Rat, Opossum (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide NKASPPFPLISNAQ-C from the N-terminus of human MPP5 (NP_071919.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Bovine, Dog, Elephant, Panda, Horse, Rabbit, Pig (100%), Bat, Hamster, Turkey, Chicken (93%), Rat, Opossum (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MPP5

Target: MPP5 (Membrane Protein, Palmitoylated 5 (MAGUK P55 Subfamily Member 5) (MPP5))

Alternative Name: MPP5

Background: Name/Gene ID: MPP5

Synonyms: MPP5, MAGUK p55 subfamily member 5, Stardust, PALS1

Gene ID: 64398

NCBI Accession: NP_071919

UniProt: Q8N3R9

Pathways: Nucleotide Phosphorylation, Tube Formation, Asymmetric Protein Localization