KPNA3 antibody (AA 509-521)

Catalog No. ABIN337019

The Goat Polyclonal anti-KPNA3 antibody is suitable to detect KPNA3 in samples from Human, Mouse, Rat, Cow, Dog, Hamster, Monkey, Pig, Bat, Chicken, Horse and Rabbit. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for KPNA3 antibody (AA 509-521) (ABIN337019)

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Hamster, Monkey, Pig, Bat, Chicken, Horse, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This KPNA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KPNA3 Antibody

Binding Specificity: AA 509-521

Specificity: Human KPNA3.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DPTANLQTKEFNF from the C-terminus of human KPNA3 (NP_002258.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA, WB (0.1 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KPNA3

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Alternative Name: KPNA3 / Importin Alpha 4

Background: Name/Gene ID: KPNA3

Synonyms: KPNA3, Importin alpha-3, Importin alpha Q2, Karyopherin subunit alpha-3, Importin-alpha-Q2, IPOA4, Qip2, SRP1, HSRP1, Importin alpha 4, Importin subunit alpha-3, SRP1-gamma, SRP1gamma, SRP4

Gene ID: 3839

NCBI Accession: NP_002258

UniProt: O00505

Pathways: Protein targeting to Nucleus