Arylsulfatase B antibody (AA 359-372)

Catalog No. ABIN337028

The Goat Polyclonal anti-Arylsulfatase B antibody is suitable to detect Arylsulfatase B in samples from Human and Monkey. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for Arylsulfatase B antibody (AA 359-372) (ABIN337028)

Target: Arylsulfatase B (ARSB)

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Arylsulfatase B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Arylsulfatase B Antibody

Binding Specificity: AA 359-372

Specificity: Human ARSB. This antibody is expected to recognise both reported isoforms (NP_000037.2, NP_942002.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Elephant (93%) Monkey, Bovine, Bat, Cat, Panda, Horse, Chicken (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KLARGHTNGTKPLD from an internal region of human ARSB (NP_000037.2, NP_942002.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%), Elephant (93%), Monkey, Bovine, Bat, Cat, Panda, Horse, Chicken (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Arylsulfatase B

Target: Arylsulfatase B (ARSB)

Alternative Name: ARSB / Arylsulfatase B

Background: Name/Gene ID: ARSB

Synonyms: ARSB, Arylsulfatase B, ASB, G4S, MPS6

Gene ID: 411

NCBI Accession: NP_000037, NP_942002

UniProt: P15848

Pathways: Glycosaminoglycan Metabolic Process