DARC antibody (C-Term)

Catalog No. ABIN337034

The Rabbit Polyclonal anti-DARC antibody is suitable to detect DARC in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for DARC antibody (C-Term) (ABIN337034)

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DARC Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (2.5 - 5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Alternative Name: ACKR1

Background: Name/Gene ID: ACKR1
Subfamily: Chemokine
Family: GPCR

Synonyms: ACKR1, CD234, CD234 antigen, DARC, Duffy antigen, Duffy blood group, Duffy group antigen, Dfy, FY, Fy glycoprotein, Plasmodium vivax receptor, WBCQ1, CCBP1, Duffy blood group antigen, Glycoprotein D, GPD, GpFy

Gene ID: 2532