GCLC antibody (AA 295-313)

Catalog No. ABIN337056

The Rabbit Polyclonal anti-GCLC antibody (ABIN337056) specifically detects GCLC in WB, IHC, ELISA, IP and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow and Monkey samples.

Quick Overview for GCLC antibody (AA 295-313) (ABIN337056)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Human, Mouse, Rat, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GCLC Antibody

Binding Specificity: AA 295-313

Specificity: Recognizes the heavy subunit of rat GCS. Species cross-reactivity: human, monkey and mouse. Cross-reacts weakly with bovine.

Purification: Protein A purified

Immunogen: A synthetic peptide derived from corresponding to aa295-313 of rat GCLC (heavy subunit of GCS).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 2.5-5 μg/mL for 2hrs at RT. Immunoprecipitation: 10 μg/mg protein lysate. Denatured verified. Use Protein A. Positive control: Jurkat, MAD109, PC12 cells.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC

Background: Name/Gene ID: GCLC

Synonyms: GCLC, GCL, GCS heavy chain, Gamma-ECS, GCS, GLCL, GLCLC

Gene ID: 2729

UniProt: P48506

Pathways: Cell RedoxHomeostasis