SLC12A4 antibody (AA 997-1007)

Catalog No. ABIN337105

This Goat Polyclonal antibody specifically detects SLC12A4 in ELISA, IHC, WB and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Dog, Bat and Hamster.

Quick Overview for SLC12A4 antibody (AA 997-1007) (ABIN337105)

Target: SLC12A4 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))

Reactivity: Human, Rat, Mouse, Dog, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC12A4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SLC12A4 Antibody

Binding Specificity: AA 997-1007

Specificity: Human SLC12A4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Dog, Bat (100%) Marmoset, Panda, Bovine, Rabbit, Horse (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DPSHAPDNFRE from an internal region of human SLC12A4 (NP_005063.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Dog, Bat (100%), Marmoset, Panda, Bovine, Rabbit, Horse (91%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:4000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SLC12A4

Target: SLC12A4 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))

Alternative Name: SLC12A4 / KCC1

Background: Name/Gene ID: SLC12A4
Subfamily: Cation:chloride symporter
Family: Transporter

Synonyms: SLC12A4, Erythroid K-Cl cotransporter 1, HKCC1, KCC1, K-Cl cotransporter, Erythroid K:Cl cotransporter

Gene ID: 6560

NCBI Accession: NP_005063

UniProt: Q9UP95