PRRX1 antibody (AA 73-83)

Catalog No. ABIN337108

This Goat Polyclonal antibody specifically detects PRRX1 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Chicken, Dog, Rabbit, Horse, Pig and Hamster.

Quick Overview for PRRX1 antibody (AA 73-83) (ABIN337108)

Target: PRRX1 (Paired Related Homeobox 1 (PRRX1))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Rabbit, Horse, Pig, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This PRRX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PRRX1 Antibody

Binding Specificity: AA 73-83

Specificity: Human PRRX1. This antibody is expected to recognise both reported isoforms (NP_008833.1, NP_073207.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Chicken (100%) Marmoset, Elephant (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-GSDTPQQDNDQ from an internal region of human PRRX1 (NP_008833.1, NP_073207.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Chicken (100%), Marmoset, Elephant (91%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:4000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PRRX1

Target: PRRX1 (Paired Related Homeobox 1 (PRRX1))

Alternative Name: PRX-1 / PRRX1

Background: Name/Gene ID: PRRX1

Synonyms: PRRX1, AGOTC, Homeobox protein PHOX1, Paired related homeobox 1, PHOX1, PRX1, Paired mesoderm homeo box 1, PRX-1, PMX1

Gene ID: 5396

NCBI Accession: NP_008833, NP_073207