s100a4 antibody (AA 86-98)

Catalog No. ABIN337109

The Goat Polyclonal anti-s100a4 antibody is suitable to detect s100a4 in samples from Human, Cow, Rabbit, Dog, Horse and Pig. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for s100a4 antibody (AA 86-98) (ABIN337109)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivity: Human, Cow, Rabbit, Dog, Horse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-s100a4 Antibody

Binding Specificity: AA 86-98

Specificity: Human S100A4 / FSP1. Both variants (NP_002952.1, NP_062427.1) represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Marmoset, Hamster, Elephant, Bat (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CNEFFEGFPDKQP from the C-terminus of human S100A4 / FSP1 (NP_002952.1, NP_062427.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Marmoset, Hamster, Elephant, Bat (92%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:128000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4 / FSP1

Background: Name/Gene ID: S100A4

Synonyms: S100A4, 42A, 18A2, Fibroblast-specific protein-1, FSP1, MTS1, PEL98, Protein Mts1, Calvasculin, CAPL, Metastasin, p9KA, Protein S100-A4

Gene ID: 6275

NCBI Accession: NP_002952, NP_062427

UniProt: P26447

Pathways: S100 Proteins