XBP1 antibody (AA 358-372)

Catalog No. ABIN337110

The Goat Polyclonal anti-XBP1 antibody (ABIN337110) specifically detects XBP1 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human and Monkey samples.

Quick Overview for XBP1 antibody (AA 358-372) (ABIN337110)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-XBP1 Antibody

Binding Specificity: AA 358-372

Specificity: Human XBP1. This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Panda (93%) Pig, Opossum (87%) Bat, Turkey, Pufferfish (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NHSWEDTFANELFPQ from an internal region of human XBP1 (NP_001073007.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Panda (93%), Pig, Opossum (87%), Bat, Turkey, Pufferfish (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:4000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1

Background: Name/Gene ID: XBP1

Synonyms: XBP1, X-box binding protein 1, X-box-binding protein 1, XBP2, TREB5, XBP-1

Gene ID: 7494

NCBI Accession: NP_001073007

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response