CK1 epsilon antibody (AA 403-416)

Catalog No. ABIN337139

The Goat Polyclonal anti-CK1 epsilon antibody is suitable to detect CK1 epsilon in samples from Human. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for CK1 epsilon antibody (AA 403-416) (ABIN337139)

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CK1 epsilon antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CK1 epsilon Antibody

Binding Specificity: AA 403-416

Specificity: Human CSNK1E. Both reported variants represent identical protein (NP_001885.1 and NP_689407.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Turkey, Chicken, Platypus (93%) Xenopus (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PASQTSVPFDHLGK from the C-terminus of human CSNK1E (NP_001885.1, NP_689407.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Turkey, Chicken, Platypus (93%), Xenopus (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Alternative Name: CSNK1E / CK1 Epsilon

Background: Name/Gene ID: CSNK1E
Subfamily: Casein kinase I
Family: Protein Kinase

Synonyms: CSNK1E, Casein kinase 1 epsilon, Casein kinase i epsilon, CK1 epsilon, CKIE, CKIepsilon, CKI-epsilon, HCKIE, Casein kinase 1, epsilon

Gene ID: 1454

NCBI Accession: NP_001885, NP_689407

UniProt: P49674

Pathways: Hedgehog Signaling, M Phase