APOA4 antibody (AA 359-371)

Catalog No. ABIN337144

This Goat Polyclonal antibody specifically detects APOA4 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for APOA4 antibody (AA 359-371) (ABIN337144)

Target: APOA4 (Apolipoprotein A-IV (APOA4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOA4 Antibody

Binding Specificity: AA 359-371

Specificity: Human APOA4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon, Baboon, Monkey, Galago (92%) Marmoset, Armadillo (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KEKESQDKTLSLP from an internal region (near the C-terminus) of human APOA4 (NP_000473.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Baboon, Monkey, Galago (92%), Marmoset, Armadillo (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APOA4

Target: APOA4 (Apolipoprotein A-IV (APOA4))

Alternative Name: Apolipoprotein A4 / APOA4

Background: Name/Gene ID: APOA4

Synonyms: APOA4, Apo-AIV, Apolipoprotein A-IV, Apolipoprotein A4, ApoA-IV

Gene ID: 337

NCBI Accession: NP_000473

UniProt: P06727

Pathways: Lipid Metabolism