PDLIM1 antibody (AA 316-329)

Catalog No. ABIN337145

The Goat Polyclonal anti-PDLIM1 antibody is suitable to detect PDLIM1 in samples from Human. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for PDLIM1 antibody (AA 316-329) (ABIN337145)

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PDLIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDLIM1 Antibody

Binding Specificity: AA 316-329

Specificity: Human PDLIM1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Rat, Hamster, Panda, Bat, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Zebrafish (93%) Elephant, Bovine, Pig, Stickleback (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TPPEGYEVVTVFPK from the C-terminus of human PDLIM1 (NP_066272.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Rat, Hamster, Panda, Bat, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Zebrafish (93%), Elephant, Bovine, Pig, Stickleback (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PDLIM1

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Alternative Name: PDLIM1

Background: Name/Gene ID: PDLIM1

Synonyms: PDLIM1, CLP-36, CLP36, CLIM1, Elfin, ENIGMA, HCLIM1, PDZ and LIM domain 1, Lim, PDZ and LIM domain 1 (elfin), PDZ and LIM domain protein 1, LIM domain protein CLP-36

Gene ID: 9124

NCBI Accession: NP_066272

UniProt: O00151