CCR6 antibody (AA 18-46)

Catalog No. ABIN337207

This Goat Polyclonal antibody specifically detects CCR6 in ELISA, FACS, IHC, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for CCR6 antibody (AA 18-46) (ABIN337207)

Target: CCR6 (Chemokine (C-C Motif) Receptor 6 (CCR6))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCR6 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-CCR6 Antibody

Binding Specificity: AA 18-46

Specificity: Recognizes human CCR6. Peptide sequence is <50 % identical to other human chemokine receptors in this region. Species cross-reactivity: mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa 18-46 (Y18FVSVNTSYYSVDSEMLLCSLQEVRQFSR46) of human CCR6 (C-C chemokine receptor 6). Peptide sequence is <50% identical to other human chemokine receptors in this region. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:75000), Flo (1 - 3 μg/10 x10⁶), ICC (1:300), IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA: 1:75000. Flow Cytometry: 50-100 μL, 1-3 μL per 10^6 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CCR6

Target: CCR6 (Chemokine (C-C Motif) Receptor 6 (CCR6))

Alternative Name: CCR6

Background: Name/Gene ID: CCR6
Subfamily: Chemokine
Family: GPCR

Synonyms: CCR6, C-c chemokine receptor 6, CD196 antigen, BN-1, C-C chemokine receptor type 6, C-C CKR-6, Chemokine c-c motif receptor 6, CKRL3, CMKBR6, Chemokine (C-C) receptor 6, Chemokine receptor-like 3, Cc chemokine receptor 6, CC-CKR-6, CCR-6, DCR2, DRY6, G protein-coupled receptor 29, DRY-6, G-protein coupled receptor 29, GPR29, GPRCY4, Strl-22, STRL22, CD196, CKR-L3, GPR-CY4, LARC receptor

Gene ID: 1235

UniProt: P51684

Pathways: cAMP Metabolic Process