TAO Kinase 3 antibody (AA 396-411)

Catalog No. ABIN337220

The Goat Polyclonal anti-TAO Kinase 3 antibody is suitable to detect TAO Kinase 3 in samples from Human, Cow, Horse, Rabbit, Bat and Pig. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for TAO Kinase 3 antibody (AA 396-411) (ABIN337220)

Target: TAO Kinase 3 (TAOK3)

Reactivity: Human, Cow, Horse, Rabbit, Bat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This TAO Kinase 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TAO Kinase 3 Antibody

Binding Specificity: AA 396-411

Specificity: Human TAOK3.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Bovine, Bat, Horse, Rabbit, Pig (100%) Marmoset, Mouse, Rat, Hamster, Panda (94%) Opossum (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HKKDHVFIRDEAGHGD from an internal region of human TAOK3 (NP_057365.3). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Bovine, Bat, Horse, Rabbit, Pig (100%), Marmoset, Mouse, Rat, Hamster, Panda (94%), Opossum (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2 - 3 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:5.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TAO Kinase 3

Target: TAO Kinase 3 (TAOK3)

Alternative Name: TAOK3 / JIK

Background: Name/Gene ID: TAOK3
Subfamily: TAO
Family: Protein Kinase

Synonyms: TAOK3, DPK, JNK/SAPK-inhibitory kinase, Jun kinase-inhibitory kinase, KDS, MAP3K18, Ste20-like kinase, TAO3, TAO kinase 3, HKFC-A, JIK, Kinase from chicken homolog A

Gene ID: 51347

NCBI Accession: NP_057365

UniProt: Q9H2K8