ALDH1A1 antibody (AA 476-488)

Catalog No. ABIN337223

The Goat Polyclonal anti-ALDH1A1 antibody is suitable to detect ALDH1A1 in samples from Human, Cow and Sheep. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for ALDH1A1 antibody (AA 476-488) (ABIN337223)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human, Cow, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ALDH1A1 Antibody

Binding Specificity: AA 476-488

Specificity: Human ALDH1A1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Sheep, Bovine (100%) Marmoset, Bat, Horse, Opossum, Lizard (92%) Dog, Chicken, Platypus, Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RELGEYGFHEYTE from an internal region of human ALDH1A1 (NP_000680.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Sheep, Bovine (100%), Marmoset, Bat, Horse, Opossum, Lizard (92%), Dog, Chicken, Platypus, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1 / ALDH1

Background: Name/Gene ID: ALDH1A1

Synonyms: ALDH1A1, Acetaldehyde dehydrogenase 1, ALDH class 1, ALDC, Aldehyde dehydrogenase 1, ALDH-E1, ALDH1, ALDH11, PUMB1, RALDH 1, RALDH1, ALHDII, Retinal dehydrogenase 1, Retinaldehyde dehydrogenase 1

Gene ID: 216

NCBI Accession: NP_000680

UniProt: P00352

Pathways: Dopaminergic Neurogenesis