GAPDH antibody (AA 73-87)

Catalog No. ABIN337259

The Rabbit Polyclonal anti-GAPDH antibody is suitable to detect GAPDH in samples from Human and Monkey. It has been validated for WB, IHC and IHC (p).

Quick Overview for GAPDH antibody (AA 73-87) (ABIN337259)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAPDH Antibody

Binding Specificity: AA 73-87

Specificity: Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein was used as the immunogen.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset (100%) Pig (93%) Mouse, Rat, Cat, Chicken, Armadillo, Xenopus (87%).

Purification: Protein G purified

Immunogen: Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset (100%), Pig (93%), Mouse, Rat, Cat, Chicken, Armadillo, Xenopus (87%).

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Name/Gene ID: GAPDH

Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

Gene ID: 2597

UniProt: P04406