Thrombospondin I (THBS1) antibody

Antigen: Thrombospondin I (THBS1)

Synonyms: TMTSP, UNQ3010, MGC74971, Tmtsp, AW121720, 4833423O18Rik

Clonality: Monoclonal (A6.1)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN337292

Additional Information

Alternative name: Thrombospondin

Immunogen: Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.

Isotype: IgG1  (Matching secondary antibodies)

Clone: A6.1

Description: Gene: Thrombospondin

Specificity: Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat. Predicted Reactivities: Cow (Bovine),Dog (Canine),Horse (Equine),Mouse (Murine),Pig (Porcine),Rat (Rattus),Sheep (Ovine).

Application Details

Application Notes: Flo, IF, IHC-P (10 µg/ml), IP, WB

Concentration: 1 mg/ml

Purification: Protein G Column

Buffer: 10 mM PBS, pH 7.4.

Storage: Long term: -20°C, Short term: +4°C

Research Area: Cancer, Angiogenesis, Glia marker, Extracellular Matrix

Restrictions: For Research Use only

Images

Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with THBS1 antibody ABIN337292 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.