FZD7 antibody (AA 559-572)

Catalog No. ABIN337331

The Goat Polyclonal anti-FZD7 antibody is suitable to detect FZD7 in samples from Human, Mouse, Rat, Dog, Cow, Rabbit, Bat, Monkey and Hamster. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for FZD7 antibody (AA 559-572) (ABIN337331)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Bat, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FZD7 Antibody

Binding Specificity: AA 559-572

Specificity: Human FZD7 / Frizzled 7.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Opossum (100%) Platypus (93%) Chicken, Xenopus (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RFYHRLSHSSKGET from the C-terminus of human FZD7 / Frizzled 7 (NP_003498.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Opossum (100%), Platypus (93%), Chicken, Xenopus (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: FZD7 / Frizzled 7

Background: Name/Gene ID: FZD7
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD7, Frizzled family receptor 7, Frizzled homolog 7, Frizzled 7, Frizzled-7, FzE3, Fz-7, HFz7

Gene ID: 8324

NCBI Accession: NP_003498

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance