ARIH1 antibody (AA 513-526)

Catalog No. ABIN337340

The Goat Polyclonal anti-ARIH1 antibody is suitable to detect ARIH1 in samples from Human, Mouse, Dog, Zebrafish (Danio rerio), Cow, Horse, Rabbit, Rat, Bat, Chicken, Monkey, Pig and Xenopus laevis. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for ARIH1 antibody (AA 513-526) (ABIN337340)

Target: ARIH1 (Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (ARIH1))

Reactivity: Human, Mouse, Dog, Zebrafish (Danio rerio), Cow, Horse, Rabbit, Rat, Bat, Chicken, Monkey, Pig, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This ARIH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARIH1 Antibody

Binding Specificity: AA 513-526

Specificity: Human ARIH1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HEGYEKDLWEYIED from the C-terminus of human ARIH1 (NP_005735.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ARIH1

Target: ARIH1 (Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (ARIH1))

Alternative Name: ARIH1

Background: Name/Gene ID: ARIH1

Synonyms: ARIH1, ARI, HARI, Monocyte protein 6, MOP-6, Protein ariadne-1 homolog, UbcH7-binding protein, ARI-1, UbcM4-interacting protein, H7-AP2, HHARI, MOP6, UBCH7BP

Gene ID: 25820

NCBI Accession: NP_005735

UniProt: Q9Y4X5