Human Adenovirus type 2/5 (HAdV-2/5) antibody

Catalog No. ABIN337510

This Mouse Monoclonal antibody specifically detects Human Adenovirus type 2/5 in ICC, IP, ELISA and WB. It exhibits reactivity toward Human Adenovirus 2 and Human Adenovirus 5.

Quick Overview for Human Adenovirus type 2/5 (HAdV-2/5) antibody (ABIN337510)

Target: Human Adenovirus type 2/5 (HAdV-2/5)

Reactivity: Human Adenovirus 2, Human Adenovirus 5

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)

Clone: M73

Product Details

Specificity: Recognizes the three adenovirus (Ad) 2 and 5 E1A gene products, Mr 36-50kD.

Purification: Protein A purified

Immunogen: TrpE-Ad2 E1A fusion protein isolated from E. coli.

Type of Immunogen: Fusion protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, ICC (0.1 μg/mL), IP, WB

Usage: Suitable for use in ELISA, Immunoprecipitation, Immunocytochemistry and Western Blot. Western Blot Analysis: 0.05-0.1 μg/mL detects the Ad2 and Ad5 E1A gene products in RIPA lysates from 293 cells. 293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- E1A (Ad2/Ad5) (0.1 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: An independent laboratory has reported that this antibody immunoprecipitates Ad2 and Ad5 E1A gene products and associated proteins. Immunocytochemistry: An independent laboratory has reported that this antibody immunostains Ad2 and Ad5 E1A gene products.

Comment:

Target Species of Antibody: Adenovirus

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 M sodium phosphate,  pH 7.4, 0.1 % sodium azide, 0.2 % gelatin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details

Target: Human Adenovirus type 2/5 (HAdV-2/5)

Target Type: Virus