Goat anti-Rabbit IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN338968

Quick Overview for Goat anti-Rabbit IgG (F(ab')2 Region) Antibody (Alkaline Phosphatase (AP)) (ABIN338968)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Rabbit

Host: Goat

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG, Rabbit IgG F (ab')2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c).

Purification: Immunoaffinity purified

Immunogen: Rabbit IgG F (ab')2 fragment.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, IHC, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilution (s): This product has been assayed against 1 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:4500 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details

Target: IgG

Target Type: Antibody