Goat anti-Rabbit IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))
Quick Overview for Goat anti-Rabbit IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) (ABIN338983)
Target
Clonality
Conjugate
Application
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Binding Specificity
- Fc Region
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Reactivity
- Rabbit
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Host
- Goat
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Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F (ab')2 or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
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Purification
- Immunoaffinity purified
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Immunogen
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Rabbit IgG F (ab')2 fragment.
Type of Immunogen: Purified protein
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Application Notes
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Approved: ELISA (1:600), IHC, WB
Usage: Suitable for use in Western Blot (Western or Dot Blot), ELISA and Immunohistochemistry. ELISA: 1:600 to 1:2800. 1.0 μg of rabbit IgG was used in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Comment
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Target Species of Antibody: Rabbit
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M Magnesium chloride, 0.0001 M Zinc chloride, pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide, 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. -
Storage
- 4 °C
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Storage Comment
- Store at 4°C. Do not freeze.
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- IgG
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Target Type
- Antibody
Target
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