Phosphothreonine antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Application
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 Alternatives Western Blotting (WB), Immunoprecipitation (IP), ELISA, Functional Studies (Func)
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| Catalog no. |
ABIN340104 |
| Quantity |
100 µg |
| Price |
626.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Immunogen
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Purified protein, Keyhole Limpet Hemocyanin (KLH)- conjugated phosphothreonine conjugates and Phosvitin.
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Isotype
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IgG2a, kappa chain (Matching secondary antibodies)
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Description
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Gene: Phosphothreonine
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Specificity
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Recognizes Phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins. Binding of Ly-pT-Gly-HRP (0.2 ug/100 ul/well) to an anti-phosphothreonine coated microplate (0.5 ug/100 ul/well) is inhibited by 95% with the addition of 2-10 ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/ml. Species cross-reactivity: species independent.
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Application Notes
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ELISA (0.5 µg/ml), Func, IP, WB (2 µg/ml)
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Purification
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Immunoaffinity Chromatography
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Buffer
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PBS, pH 7.4, 0.1% sodium azide, 40% glycerol.
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Research Area
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Signaling, Metabolism, Amino Acids, Protein Modifications
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Restrictions
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For Research Use only
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Alternatives for antigen "Phosphothreonine", type "Antibodies"
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Hosts
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Rabbit (34), Mouse (14)
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Reactivities
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Human (4), Dog (Canine) (3), Mouse (Murine) (3), Rat (Rattus) (3), All Species (2)
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Applications
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Western Blotting (WB) (45), ELISA (27), Immunoprecipitation (IP) (22), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (IHC) (2), Flow Cytometry (FACS) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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HRP (9), Biotin (7)
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