Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta) antibody

Antigen: Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta)

Clonality: Monoclonal (MBH90AB)

Host: Mouse

Reactivity: Human, Mouse (Murine), Cow (Bovine)

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN343706

Additional Information

Alternative name: Hsp90 alpha,beta

Immunogen: Peptide corresponding to the sequence EEVHHGEEEVEC within N-terminal part of humanHsp90.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MBH90AB

Description: Hsp90 (heat shock protein 90) is one of the most abundant chaperones in the cytosol ofeukaryotic cells. It interacts with various proteins, including protein kinases and transcriptionfactors, and either facilitates their stabilization and activation or directs them for proteasomaldegradation. Hsp90 thus affects multiple signaling pathways and biological processes andmodulation of this single target offers the prospect of simultaneous intervence to various keypoints of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycledriven by ATP binding and hydrolysis. There are two isoforms, alpha and beta, of vertebrateHsp90. Whereas Hsp90 beta is expressed constitutively to a high level, Hsp90 alpha isstress-inducible and is overexpressed in many cancerous cells.

Specificity: The antibody MBH90AB recognizes the epitope EEEVE within N-terminal part ofubiquitously expressed Hsp90 alpha and Hsp90 beta proteins with calculated Mw of 84.7kDa and 83.3 kDa, respectively, however, migrating as 90 kDa bands under reducingSDS-PAGE conditions.

Application Details

Application Notes: Immunoprecipitation - Recommended dilution: 1 µg/ml. Western Blotting - Recommended dilution: 1 µg/ml

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western blotting analysis of Hsp90Alpha and Beta protein by antibody MBH90AB to both Hsp90Alpha and Beta isoform.