BRG1 antibody

Antigen: BRG1

Clonality: Monoclonal (BRG-01)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN343708

Additional Information

Immunogen: Bacterially produced GST-fused protein representing 242 C-terminal amino acids of humanBrg1.

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: BRG-01

Description: Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-likechromatin-remodeling complexes that enable access of regulatory and effector proteins intranscription, DNA repair and DNA replication. Although Brg1-containing complexes are notessential for general cell survival, they participate in transcriptional regulation of severalhundred genes including those involved in interferon and stress response, immune cellsdifferentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouseembryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumoursupression.

Specificity: The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.

Application Details

Application Notes: Western Blotting - Recommended dilution: 10 µg/ml. Positive control: HEK293 cells

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer, Wnt, Neural Stem Cell marker

Restrictions: For Research Use only

Images

Immunostaining of Brg1 by BRG-01 antibody.A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression.B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.