SMARCA4 antibody (C-Term)

Catalog No. ABIN343708

This Mouse Monoclonal antibody specifically detects SMARCA4 in WB. It exhibits reactivity toward Human.

Quick Overview for SMARCA4 antibody (C-Term) (ABIN343708)

Target: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMARCA4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: BRG-01

Product Details anti-SMARCA4 Antibody

Binding Specificity: C-Term

Purpose: Anti-BRG1 Purified

Cross-Reactivity (Details): Human, Other not determined

Characteristics: The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.

Isotype: IgG2a

Application Details

Application Notes: Western blotting: Recommended dilution: 10 μg/mL, positive control: HEK293 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for SMARCA4

Target: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Alternative Name: BRG1

Background: SWI/SNF related, matrix associated, actin dependen,Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.,CSS4, SMARCA4, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB

Gene ID: 6597

UniProt: P51532

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance