AGR3 antibody
| Antigen |
|
| Clonality |
Monoclonal (AGR3-1) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
|
| Catalog no. |
ABIN343711 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
403.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 3 to 5 Business days |
|
Immunogen
|
Purified human AGR3 protein
|
|
Format
|
Purified
|
|
Isotype
|
IgG1 (Matching secondary antibodies)
|
|
Clone
|
AGR3-1
|
|
Description
|
AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), orBCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction andp53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutictarget for hormone-responsive breast tumours. Its Xenopus homolog is associated withanteroposterior fate determination during early development.
|
|
Specificity
|
The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein(19-20 kDa), a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.
|
|
Application Notes
|
Western Blotting - Recommended dilution: 1 µg/ml. Positive control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line. Immunohistochemistry (paraffin sections) - Recommended dilution: 1 µg/ml. Positive tissue: breast cancerImmunohistochemistry (frozen sections) - Recommended dilution: 1 µg/ml. Positive tissue: breast cancer. Immunocytochemistry - Recommended dilution: 1 µg/ml. Positive control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line
|
|
Concentration
|
1 mg/ml
|
|
Purity
|
> 95% (by SDS-PAGE)
|
|
Purification
|
Purified from ascites by protein-A affinity chromatography.
|
|
Buffer
|
The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
|
|
Storage
|
Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "AGR3", type "Antibodies"
|
Hosts
|
Mouse (5), Rabbit (2)
|
|
Reactivities
|
Human (7), Mouse (Murine) (1)
|
|
Applications
|
Western Blotting (WB) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Flow Cytometry (FACS) (2), Immunohistochemistry (IHC) (2), ELISA (1), ELISA (Detection) (1), Immunofluorescence (IF) (1)
|
