AGR2+AGR3 antibody

Antigen: AGR2+AGR3

Clonality: Monoclonal (AGR3-4)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN343713

Additional Information

Immunogen: Purified human AGR3 protein

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: AGR3-4

Description: AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, andAGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, aresecreted cytoplasmic proteins which are involved in metastasis induction and p53 tumoursupressor inhibition. They may serve as molecular markers and potential therapeutic targetsfor hormone-responsive breast tumours, AGR2 was reported also as a marker of othercarcinomas. Xenopus homolog of these proteins is associated with anteroposterior fatedetermination during early development.

Specificity: The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2)proteins (19-20 kDa), secreted cytoplasmic proteins which can serve as markers ofcarcinogenesis.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line. Immunocytochemistry - Recommended dilution: 1 µg/ml. Positive control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.