CD9 (CD9) antibody
| Antigen |
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| Synonyms |
5H9, BA2, P24, GIG2, MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29 |
| Clonality |
Monoclonal (EM-04) |
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Host
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Reactivity
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Human (121), Mouse (Murine) (25), Cow (Bovine) (16), Cat (Feline) (15), Dog (Canine) (13), Rabbit (13), Horse (Equine) (12), Rat (Rattus) (8), Monkey (7), Pig (Porcine) (7), Sheep (Ovine) (1)
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)
Flow Cytometry (FACS) (122), Western Blotting (WB) (55), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (30), Immunoprecipitation (IP) (26), Functional Studies (Func) (17), Immunofluorescence (IF) (13), Immunohistochemistry (IHC) (9), Enzyme Immunoassay (EIA) (6), ELISA (4), Immunocytochemistry (ICC) (3), Blocking Antibody (Inhibition) (1)
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| Catalog no. |
ABIN343715 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
208.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD9
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Immunogen
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Permeabilized murine bone marrow-derived mast cells (BMMC).
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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EM-04
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Description
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CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associatedtetraspanin-enriched signaling microdomains within the plasma membrane, formingcomplexes with each other as well as with integrins, membrane-anchored growth factorsand other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth,myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferationand is necessary for association of heterologous MHC II molecules on the dendritic cellplasma membrane which is important for effective T cell stimulation. CD9 is also consideredas metastasis suppressor in solid tumors.
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Specificity
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The rat monoclonal antibody EM-04 recognizes CD9 antigen, a 24 kDa transmembraneprotein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activatedT lymphocytes.
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Application Notes
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Flow Cytometry: Recommended dilution: 4 µg/ml. - Recommended dilution: 1 µg/ml. Application note: non-reducing conditions
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from hybridoma culture supernatant by hydrophobic charge inductionchromatography followed by precipitation methods.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Embryogenesis, Embryonic stem cells, Reproduction, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD9 (CD9)", type "Antibodies"
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Hosts
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Mouse (116), Rat (22), Rabbit (18)
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Reactivities
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Human (121), Mouse (Murine) (25), Cow (Bovine) (16), Cat (Feline) (15), Dog (Canine) (13), Rabbit (13), Horse (Equine) (12), Rat (Rattus) (8), Monkey (7), Pig (Porcine) (7), Sheep (Ovine) (1)
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Applications
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Flow Cytometry (FACS) (122), Western Blotting (WB) (55), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (30), Immunoprecipitation (IP) (26), Functional Studies (Func) (17), Immunofluorescence (IF) (13), Immunohistochemistry (IHC) (9), Enzyme Immunoassay (EIA) (6), ELISA (4), Immunocytochemistry (ICC) (3), Blocking Antibody (Inhibition) (1)
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Conjugates
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FITC (20), PE (20), Biotin (11), APC (4), RPE (3), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Pacific Blue (1)
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Epitopes
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Center (2), N-Term (2)
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