Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 4 (LGR4) antibody

Antigen: Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 4 (LGR4)

Synonyms: GPR48

Clonality: Monoclonal (6G8-B3-G5-C3)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN349637

Additional Information

Alternative name: LGR4

Gene ID: NP_060960

Swiss-Prot: Q8N537

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Format: Liquid (sterile filtered)

Isotype: IgG2b kappa  (Matching secondary antibodies)

Clone: 6G8-B3-G5-C3

Description: LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

Specificity: This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human LGR4 protein. A BLAST analysis was used to suggest cross-reactivity with LGR4 from chimpanzee, orangutan and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with LGR4 from other sources has not been determined.

Application Details

Application Notes: Recommended Dilutions: ELISA 1:20,000 WESTERN BLOT 1:500 - 1:3,000 IMMUNOHISTOCHEMISTRY 5 µg/ml OTHER APPLICATIONS User Optimized. Note: This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

Concentration: 1.13 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

Immunohistochemistry. Anti-LGR4 monoclonal antibody was used diluted to 5 µg/ml to detect LGR4 staining at the membrane of cells in various human tissues. A. Brain cerebellum. B. Pancreas islet. Strongly positive staining is noted in subsets of cells within the islets of Langerhans. Moderately positive staiing was observed in Purkinje and Golgi neurons of the cerebellum, adrenal medulla, neuroendocrine cells, hepatocytes, lung macrophages, seminiferous tubules and Leydig cells of the testis. Faintly to moderately positive staining was also observed in cardiac myocytes and renal tubules, granulocytes, and subsets of lymphocytes. Some elastin background staining is noted. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA. A B

anti-Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 4 (LGR4) antibody (Image 2)