Alternative Oxidase 1/2 (AOX1/AOX2) antibody

Antigen: Alternative Oxidase 1/2 (AOX1/AOX2)

Synonyms: aox2, aox1, ALTERNATIVE OXIDASE 1A, AOX1A, ATAOX1A, ALTERNATIVE OXIDASE, AOX2, MSJ1.5, MSJ1_5, alternative oxidase 1D, AOX1D, F27G20.12

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Lupinus luteus, Pea (Pisum sativum), Potato (Solanum tuberosum)

Application: Western Blotting (WB)

Catalog no.: ABIN349644

Additional Information

Alternative name: Plant alternative oxidase 1 and 2 (AOX1/2)

Immunogen: KLH-conjugated synthetic peptide derived from fully conserved C-terminalconsensus motif from plant AOX isoforms 1 and 2 including Arabidopsis thalianaAOX1A (At3g22370) and AOX2 (At5g64210), Solanum lycopersicum Q7XBG9,Oryza sativa Q7XT33,

Format: Lyophilized

Description: Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrialmembrane of plants. They function as terminal oxidases in the alternate electrontransport pathway, oxidizing ubiquinone to reduce oxygen to water.

Specificity: Predicted reactivity: dicots including: Nicotiana tabacum and Glycine max, monocots including Oryzasativa, S.officinarum and Triticum aestivum, conifers and Physcomitrella patens. Not reactive in: Chlamydomonas reinhardtii.

Application Details

Application Notes: Recommended Dilution 1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection(WB).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

20ug of total protein from (2) Arabidopsis thaliana mitochondria and leafs of (1)Arabidopsis thaliana and cold-stressed (3) Solanum tuberosum and (4) Pisumsativum were separated on 4-12% NuPage (Invitrogen) and blotted onnitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1%TWEEN 20) and probed with anti-AOX (ABIN349644, 1:1000, 1h) and secondaryanti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2%low fat milk powder. Antibody incubations were followed by washings in TBS-T (15,+5, +5, +5 min). All steps were performed at RT with agitation. Signal was detectedwith standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (120s, standardsensitivity). Isolation of total leaf protein was done with PEB .

anti-Alternative Oxidase 1/2 (AOX1/AOX2) antibody (Image 2)

Publications

Publications: Yamasaki, Abdel-Ghany, Cohu et al.: "Regulation of copper homeostasis by micro-RNA in Arabidopsis." in: The Journal of biological chemistry, Vol. 282, Issue 22, pp. 16369-78, 2007 (PubMed).

Wang, Li, Liu et al.: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, 2010 (PubMed).

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, 2011 (PubMed). Method employed by authors: Western Blotting (WB)