Vacuolar Calcium-Binding Protein-Related (AtCCaP1) antibody

Antigen: Vacuolar Calcium-Binding Protein-Related (AtCCaP1)

Synonyms: T3P18.4, T3P18_4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN349667

Additional Information

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCaP1Q9SXE9

Format: Serum

Description: CCaP1 acts as a mediator in response to continuous dark or gibberellic acid.Alternative name: At1g62480/T3P18_4, T3P18.4

Specificity: Predicted reactivity: Arabidopsis thaliana.

Application Details

Application Notes: Recommended Dilution 1: 1000 with standard ECL (WB), 1: 8000 (ELISA).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

5ug (1,3) and 2.5ug (2,5) of lysate from E.coli cells expressing CCaP1 protein/lane wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-CCaP1 antibodies (ABIN349667, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publications

Publications: Ide, Tomioka, Ouchi et al.: "Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark." in: Plant & cell physiology, Vol. 48, Issue 1, pp. 54-65, 2007 (PubMed).