Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3) antibody

Antigen: Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Raphanus sativus

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN349671

Additional Information

Alternative name: PIP1 (PIP1,1, PIP1,2, PIP1,3) aquaporins

Immunogen: synthetic peptide conserved in Arabidopsis thaliana: PIP1,1 P61837, PIP1,2Q06611, PIP1,3 Q08733, PIP1,4 Q39196

Format: Serum

Description: PIPs proteins are aquaporins which facilitate the transport of water and smallneutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2,PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,

Specificity: Predicted reactivity: dicots including: Brassica sp., Vicia faba, monocots: Hordeum vulgare, Oryzasativa, trees: Juglans regia, Populus tremula.

Application Details

Application Notes: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Storage: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIPs antibodies (ABIN349671, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publications

Publications: Ishikawa, Suga, Uemura et al.: "Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana." in: FEBS letters, Vol. 579, Issue 25, pp. 5814-20, 2005 (PubMed).