Neurofilament Heavy Protein antibody

Antigen: Neurofilament Heavy Protein

Clonality: Monoclonal (NF-05)

Host: Mouse

Reactivity: Human, Pig (Porcine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Catalog no.: ABIN349701

Additional Information

Immunogen: Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: NF-05

Description: Neurofilaments (NFs) are a type of intermediate filaments (IF) expressed almost exclusivelyin neuronal cells, and in those cells most prominently in large axons. NFs in mostvertebrates are composed of three different polypeptide chains with different molecularweights neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L),which share sequence and structural similarity in a coiled-coil core domain, but differ in thelength and sequence of their N-termini and more dramatically of their C-termini which in thecase of NF-M and NF-H form the flexible extensions that link NFs to each other and to otherelements in the cytoplasm. The protein segment on the C-terminal side of the human NF-Hrod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highlycharged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine,methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequencethat covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP)greater than 40 times. Plasma neurofilament heavy chain level has been proposed as amarker of axonal injury and clinical use of its degeneration and loss has been suggested asa biomarker of several neurodegenerative diseases.

Specificity: The antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein(NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family.NF-H is mainly expressed in the central and peripheral nervous system and reproductivesystem and is biochemically very stable. This antibody does not react with: Mouse.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: Lysate of brain homogenate. ELISA: Capture antibody

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods and size-exclusion chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-05.