RHOB antibody

Catalog No. ABIN350804

The Sheep Polyclonal anti-RHOB antibody has been validated for WB and IHC. It is suitable to detect RHOB in samples from Human, Mouse and Rat.

Quick Overview for RHOB antibody (ABIN350804)

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Reactivity: Human, Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This RHOB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RHOB Antibody

Purpose: Sheep antibody to RhoB

Specificity: Specific for RhOB.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide as a part of human rat mouse Rho-related GTP-binding protein RhoB (ARH6 ARHB H6) conjugated to an immunogenic carrier protein was used as the immunogen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for RHOB

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Alternative Name: RhoB

Background: FUNCTION: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13. Interacts with RTKN. SUBCELLULAR LOCATION: Late endosome membrane, Lipid-anchor. Cell membrane, Lipid-anchor. Nucleus. Note: Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. INDUCTION: By UV irradiation N-methyl-N-nitrosourea cisplatin cyclohexamide and serum stimulation.

UniProt: P62745