RHOB antibody (Internal Region)

Catalog No. ABIN6258639

The Rabbit Polyclonal anti-RHOB antibody is suitable to detect RHOB in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ICC.

Quick Overview for RHOB antibody (Internal Region) (ABIN6258639)

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RHOB Antibody

Binding Specificity: Internal Region

Purpose: RHOB Antibody

Specificity: RHOB Antibody detects endogenous levels of total RHOB.

Predicted Reactivity: Bovine,Horse,Sheep,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RHOB, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC 1:50-1:200 IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RHOB

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Alternative Name: RHOB

Background:

Description: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.

Gene: RHOB

Molecular Weight: 22 kDa

Gene ID: 388

UniProt: P62745