MFI2 antibody (N-Term)

Catalog No. ABIN351428

The Rabbit Polyclonal anti-MFI2 antibody is suitable to detect MFI2 in samples from Human, Mouse, Rat, Horse, Monkey, Bat and Hamster. It has been validated for IHC and IHC (p).

Quick Overview for MFI2 antibody (N-Term) (ABIN351428)

Target: MFI2 (Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2))

Reactivity: Human, Mouse, Rat, Horse, Monkey, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFI2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MFI2 Antibody

Binding Specificity: N-Term

Specificity: Human MFI2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Hamster, Elephant, Panda, Horse (100%) Bovine, Rabbit, Pig (93%) Chicken, Platypus (87%) Dog, Turkey, Medaka, Zebrafish (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from N-terminus of human MFI2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Hamster, Elephant, Panda, Horse (100%), Bovine, Rabbit, Pig (93%), Chicken, Platypus (87%), Dog, Turkey, Medaka, Zebrafish (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (15 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MFI2

Target: MFI2 (Antigen P97 (Melanoma Associated) Identified By Monoclonal Antibodies 133.2 and 96.5 (MFI2))

Alternative Name: MFI2 / p97 / Melanotransferrin

Background: Name/Gene ID: MFI2

Synonyms: MFI2, Antigen p97, CD228, CD228 antigen, MAP97, Melanotransferrin, p97

Gene ID: 4241

Pathways: Transition Metal Ion Homeostasis, Cell-Cell Junction Organization