IRGM antibody (Internal Region)

Catalog No. ABIN351455

The Rabbit Polyclonal anti-IRGM antibody is suitable to detect IRGM in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for IRGM antibody (Internal Region) (ABIN351455)

Target: IRGM (Immunity-Related GTPase Family, M (IRGM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRGM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRGM Antibody

Binding Specificity: Internal Region

Specificity: IRGM antibody may recognize mouse IRGM2 and IRGM3.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IRGM

Target: IRGM (Immunity-Related GTPase Family, M (IRGM))

Alternative Name: IRGM / LRG-47

Background: Name/Gene ID: IRGM

Synonyms: IRGM, LRG-47-like protein, IFI1, Interferon-inducible protein 1, IRGM1, LRG47, LRG-47

Gene ID: 345611

UniProt: A1A4Y4