ATF7 antibody (AA 482-494)

Catalog No. ABIN351459

The Goat Polyclonal anti-ATF7 antibody (ABIN351459) specifically detects ATF7 in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Pig, Dog, Horse, Rabbit, Bat, Monkey and Hamster samples.

Quick Overview for ATF7 antibody (AA 482-494) (ABIN351459)

Target: ATF7 (Activating Transcription Factor 7 (ATF7))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Horse, Rabbit, Bat, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This ATF7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATF7 Antibody

Binding Specificity: AA 482-494

Specificity: Human ATF7. This antibody is expected to recognise all three reported isoforms (NP_001123531.1, NP_006847.1 and NP_001123532.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%) Lizard (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HVIMTPQSQSAGR from the C-terminus of human ATF7 (NP_001123531.1, NP_006847.1, NP_001123532.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%), Lizard (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATF7

Target: ATF7 (Activating Transcription Factor 7 (ATF7))

Alternative Name: ATF7

Background: Name/Gene ID: ATF7
Family: Transcription factor

Synonyms: ATF7, ATFA, Transcription factor ATF-A

Gene ID: 11016

NCBI Accession: NP_001123531, NP_006847, NP_001123532

UniProt: P17544