NIF3L1 antibody (AA 365-377)

Catalog No. ABIN351470

The Goat Polyclonal anti-NIF3L1 antibody is suitable to detect NIF3L1 in samples from Human and Monkey. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for NIF3L1 antibody (AA 365-377) (ABIN351470)

Target: NIF3L1 (NIF3 NGG1 Interacting Factor 3-Like 1 (NIF3L1))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This NIF3L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NIF3L1 Antibody

Binding Specificity: AA 365-377

Specificity: Human NIF3L1. This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1, NP_068596.2) encode the same isoform.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%) Mouse, Rat, Hamster, Panda, Bat, Dog, Horse, Turkey, Chicken (92%) Goat, Elephant, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-ILSETDRDPLQVV from the C-terminus of human NIF3L1 (NP_001129511.1, NP_068596.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%), Mouse, Rat, Hamster, Panda, Bat, Dog, Horse, Turkey, Chicken (92%), Goat, Elephant, Bovine (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NIF3L1

Target: NIF3L1 (NIF3 NGG1 Interacting Factor 3-Like 1 (NIF3L1))

Alternative Name: ALS2CR1 / NIF3L1

Background: Name/Gene ID: NIF3L1

Synonyms: NIF3L1, CALS-7, MDS015, NIF3-like protein 1, ALS2CR1

Gene ID: 60491

NCBI Accession: NP_001129511, NP_068596

UniProt: Q9GZT8