TFAP2C antibody (AA 270-281)

Catalog No. ABIN351479

The Goat Polyclonal anti-TFAP2C antibody is suitable to detect TFAP2C in samples from Human, Monkey and Rabbit. It has been validated for ELISA, IHC and IHC (p).

Quick Overview for TFAP2C antibody (AA 270-281) (ABIN351479)

Target: TFAP2C (Transcription Factor AP-2 gamma (Activating Enhancer Binding Protein 2 Gamma) (TFAP2C))

Reactivity: Human, Monkey, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This TFAP2C antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TFAP2C Antibody

Binding Specificity: AA 270-281

Specificity: Human TFAP2C / AP2 Gamma.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Rabbit (100%) Rat, Hamster, Panda, Dog, Horse (92%) Mouse, Bovine, Pig, Opossum (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NKTLEKMEKHRK from the C-terminus of human TFAP2C / AP2 Gamma (NP_003213.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Rabbit (100%), Rat, Hamster, Panda, Dog, Horse (92%), Mouse, Bovine, Pig, Opossum (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TFAP2C

Target: TFAP2C (Transcription Factor AP-2 gamma (Activating Enhancer Binding Protein 2 Gamma) (TFAP2C))

Alternative Name: TFAP2C / AP2 Gamma

Background: Name/Gene ID: TFAP2C

Synonyms: TFAP2C, AP2 Gamma, AP-2 gamma, ERF-1, ERF1, HAP-2g, Transcription factor ERF-1, TFAP2G, AP2-GAMMA, Estrogen receptor factor 1

Gene ID: 7022

NCBI Accession: NP_003213

UniProt: Q92754

Pathways: Stem Cell Maintenance