AKR1C4 antibody (AA 2-14)

Catalog No. ABIN351481

This Goat Polyclonal antibody specifically detects AKR1C4 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for AKR1C4 antibody (AA 2-14) (ABIN351481)

Target: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKR1C4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AKR1C4 Antibody

Binding Specificity: AA 2-14

Specificity: Human AKR1C4. This antibody may cross-react with AKR1C1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Bovine (92%) Marmoset, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide DPKYQRVELNDGH-C from the N-terminus of human AKR1C4 (NP_001809.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Bovine (92%), Marmoset, Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:4000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AKR1C4

Target: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Alternative Name: AKR1C4 / Chlordecone Reductase

Background: Name/Gene ID: AKR1C4

Synonyms: AKR1C4, 3-alpha-HSD, 3-alpha-HSD1, Chlordecone reductase, CDR, CHDR, DD-4, Dihydrodiol dehydrogenase 4, C11, DD4, HAKRA

Gene ID: 1109

NCBI Accession: NP_001809

UniProt: P17516

Pathways: Steroid Hormone Biosynthesis