MYC / c-Myc (C-Term) antibody

Antigen: MYC / c-Myc (C-Term)

Clonality: Monoclonal (9E10)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN351513

Additional Information

Swiss-Prot: P01106

Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-myc.

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9E10

Description: Gene: MYC / c-Myc, Standard Gene Symbol: MYC, Synonyms: MYC, bHLHe39, c-Myc, MRTL, Myc proto-oncogene, Rats1, V-myc

Specificity: The antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells

Application Details

Application Notes: Flo (1 - 5 µg/ml), IHC-P (10 µg/ml), IP (1 - 5 µg/ml), WB (0.5 - 2 µg/ml)

Concentration: 1 mg/ml

Purification: Protein G Column

Buffer: PBS, 15 mM sodium azide, approx., pH 7.4.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Human Liver (formalin-fixed, paraffin-embedded) stained with c-Myc antibody ABIN351513 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.