Cytokeratin 18 antibody

Catalog No. ABIN356295

Product Details anti-Cytokeratin 18 Antibody

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This anitbody recognises Cytokeratin 18 (CK 18). Varieties of simple epithelia including glandular but not stratified squamous epithelia are detected by this antibody.

Characteristics: Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18, Cell proliferation-inducinggene 46 protein, Keratin type I cytoskeletal 18

Purification: Purified

Immunogen: Human breast cancer PMC 42 cells. Remarks: Molecular weight of antigen: 45 kDa

Clone: CK209

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at RT. Antigen retriever like Citrate enhances the staining of FFPE tissue sections (Boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT). Immunofluorescence. Flow Cytometry. Recommended Positive Control: Human skin, breast, lung ca.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18 (KRT18 Products)

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.GI tract contain both CK 8 and CK18 but not CK 14. Cytokeratin is expressed in GI and respiratory tract, mesothelial cells, endocrine and exocrine tissues.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis