Recoverin antibody (C-Term)

Catalog No. ABIN357114

Product Details anti-Recoverin Antibody

Target: Recoverin (RCVRN)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Recoverin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Recoverin at C-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Recoverin.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blotting 1: 100 - 1: 500Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for Recoverin

Target: Recoverin (RCVRN)

Alternative Name: Recoverin (RCVRN Products)

Synonyms: RCV1 antibody, CAR antibody, S-modulin antibody, Rcv1 antibody, rcv1 antibody, rcvrn antibody, recoverin antibody, reco antibody, recoverin antibody, recoverin, gene 2 S homeolog antibody, recoverin, gene 2 L homeolog antibody, Recoverin antibody, RCVRN antibody, Rcvrn antibody, rcvrn.2.S antibody, rcvrn.2.L antibody, reco antibody

Background: Recoverin belongs to a high-affinity calcium-binding family that includes neuronal calcium sensor-1, visinin-like proteins (VILIPs), guanylate cyclase-activating proteins (GCAPs), and Kv-channel interacting proteins (KchIPs). Features common to this family include four calcium-binding EF-hand domains, and an N-terminal myristoylation sequence. This family of proteins has been implicated in a broad range of cellular signaling functions, including phototransduction and neurotransmitter release, lipid metabolism, gene expression, and ion channel regulation. Myristoylation, the post-translational addition of a fatty acid tail, has been shown to have functional significance for other calcium-binding protein family members. Recoverin is subject to the posttranslational modification of myristoylation. Binding of calcium to recoverin elicits a change in conformation that exposes the buried hydrophobic myristoyl moiety to interaction with cell membranes and other cellular proteins.Synonyms: Cancer-associated retinopathy protein, Protein CAR, RCV1, RCVRN

Molecular Weight: 22999 Da

Gene ID: 5957

NCBI Accession: NP_002894

UniProt: P35243

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction