Ubiquitination Factor E4A (UBE4A) (C-Term) antibody

Details for Product No. ABIN357482
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Antigen
Epitope
C-Term
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 1 reference available
Quantity 0.4 mL
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Catalog No. ABIN357482
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE4A.
Isotype Ig
Specificity This antibody detects UBE4A at C-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name UBE4A
Background Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze ubiquitin chain assembly in conjunction with the first three proteins.1 Human protein KIAA0126, with a predicted 1,073 amino acid residues, is a member of the novel protein family defined by ubiquitination conjugation factor E4.2 In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eucaryotes use E4-dependent proteolysis pathways for multiple cellular functions.Synonyms: KIAA0126, Ubiquitin conjugation factor E4 A
Molecular Weight 123537 Da
Gene ID 9354, 5874
UniProt Q14139
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 200. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-Ubiquitination Factor E4A (UBE4A) (C-Term) antibody anti-Ubiquitination Factor E4A (UBE4A) (C-Term) antibody
anti-Ubiquitination Factor E4A (UBE4A) (C-Term) antibody (2) anti-Ubiquitination Factor E4A (UBE4A) (C-Term) antibody (Image 2)
Background publications Nagase, Seki, Tanaka et al.: "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 2, Issue 4, pp. 167-74, 199-210, 1996 (PubMed).

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