NAE1 antibody (C-Term)

Catalog No. ABIN359509

This Rabbit Polyclonal antibody specifically detects NAE1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for NAE1 antibody (C-Term) (ABIN359509)

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-NAE1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects NAE1 (APPBP1) at C-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human APPBP1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry on paraffin sections 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for NAE1

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Alternative Name: APPBP1

Background: APPBP1 binds to the beta-amyloid precursor protein, a cell surface protein with signal-transducing properties thought to play a role in the pathogenesis of Alzheimer's disease. In addition, this protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. APPB1 is required for cell cycle progression through the S/M checkpoint.Synonyms: Amyloid beta precursor protein-binding protein 1, Amyloid protein-binding protein 1, HPP1, NAE1

Molecular Weight: 60252 Da

Gene ID: 8883, 9606

UniProt: Q13564