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LIM Domain Kinase 1 (LIMK1) (N-Term) antibody

Details for Product No. ABIN360142
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Antigen
Synonyms limk1, xlimk1, GB16654, LIMK1, LIMK, LIMK-1
Epitope
N-Term
(28), (9), (8), (7), (7), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(137), (70), (60), (12), (1), (1), (1)
Host
Rabbit
(100), (46)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(125), (61), (32), (32), (28), (17), (10), (9), (8), (4), (3)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN360142
357.50 $
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human LIMK1.
Specificity This antibody reacts to LIM Kinase 1 (LIMK1).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name LIMK1
Background LIMK1, a member of the Ser/Thr protein kinase family, may be a component of an intracellular signaling pathway and may be involved in brain development. It phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1, and this cytoplasmic protein also binds ROCK1, whic phosphorylates LIMK1 on serine and/or threonine residues. Highest expression occurs in both adult and fetal nervous systems. It is detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex, and is expressed to a lesser extent in heart and skeletal muscle. Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. This protein contains 2 LIM zinc-binding domains and 1 PDZ/DHR domain.
Alternate names: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1
Gene ID 3984
NCBI Accession 9606
UniProt P53667
Research Area Neurology, Tyrosine Kinases, Cytoskeleton, Microfilaments
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-LIM Domain Kinase 1 (LIMK1) (N-Term) antibody anti-LIM Domain Kinase 1 (LIMK1) (N-Term) antibody
anti-LIM Domain Kinase 1 (LIMK1) (N-Term) antibody (2) anti-LIM Domain Kinase 1 (LIMK1) (N-Term) antibody (Image 2)
Background publications Frangiskakis, Ewart, Morris et al.: "LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition." in: Cell, Vol. 86, Issue 1, pp. 59-69, 1996 (PubMed).

Osborne, Martindale, Scherer et al.: "Identification of genes from a 500-kb region at 7q11.23 that is commonly deleted in Williams syndrome patients." in: Genomics, Vol. 36, Issue 2, pp. 328-36, 1997 (PubMed).

Edwards, Gill: "Structural features of LIM kinase that control effects on the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 11352-61, 1999 (PubMed).

Maekawa, Ishizaki, Boku et al.: "Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase." in: Science (New York, N.Y.), Vol. 285, Issue 5429, pp. 895-8, 1999 (PubMed).

Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Validation Images
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